Home Hypoglycemic effect of wheatgrass juice in Alloxan induced diabetic rats Hypoglycemic effect of wheatgrass juice in Alloxan induced diabetic rats M. R. N. Shaikh, Majaz Quazi, Dr. R. Y. Nandedkar Wheatgrass has many characteristics desirable for health. E-mail: [email protected] Affiliation Department of Biological Sciences, School of Dentistry of Bauru, So Paulo University, Bauru, So Paulo, Brazil Alloxan-Induced Diabetes Triggers the Development of Periodontal Disease in Rats Periodontal disease in diabetic patients presents higher severity and prevalence; and increased severity of ligature-induced periodontal disease has been verified in diabetic rats. Moringa oleifera (MO) leaves are of medicinal value and industrial uses [11]. In diabetic rats treated with two doses of essential oil, a clear protective effect was observed in hepatic function and metabolism. The present study investigated the anti-diabetic effect of Delonix regia (DR) leaves extract in alloxan induced type-2 diabetic rats. Anti diabetic effect of cherries in alloxan induced diabetic rats. They exhibit a variety of biological activities. Bocca2, A.C. Batista3, G.E.P.M. The action of reactive oxygen species with a simultaneous massive increase in cytosolic calcium concentration causes rapid destruction of B cells, resulting in type 2 diabetes [22]. Analysis of the scanning data adds further empirical support for the accuracy of the linear scan method of quantitation. Besides diabetic mice models, STZ-induced rat model is also one of the earliest animal models of this condition, which is not used as commonly as the mouse model though. In fact, the administration of glibenclamide inhibited all changes caused by alloxan-induced diabetes. Currently, there is a growing interest in evaluating herbal remedies, which are seen to be less toxic and have negligible side effects [10]. Administration of either (-)epicatechin or (+)catechin (250 mg/kg, i.p. Furthermore, we have shown lack of gain in weight and lack of statistically significant difference in cytotoxicity in rats that were treated with MO and those treated with vehicle. Glucose is a major fuel for animal cells. The decrease in acidity can be determined by titrating against standard 0.01 N sodium hydroxide. A dose of 1ml of garlic juice/100g body weight (equivalent to 0.4 g/100g BW) was orally administered daily to alloxan-diabetic rats for four weeks. In addition titration method was also done on the same sample to ensure maximum accuracy. Antidiabetic activity of alcoholic stem extract of Coscinium fenestratum in streptozotocin-nicotinamide induced type 2 diabetic rats. 24,Issue. Experiment 1 (treatment with alloxan to induce type 2 diabetes). Alloxan-induced diabetes triggered liver morphological and ultrastructural changes that closely resembled human disease, ranging from steatosis to steatohepatitis and liver fibrosis. Administration of alloxan (150 mg/kg body weight, i.p.) *P<0.05 vs. control. G. Don leaves enhance pancreatic β-cell functions, stimulates insulin secretion and ameliorates other type 2 diabetes-associated complications in rats, Journal of Ethnopharmacology, 10.1016/j.jep.2016.02.018, 183, (103-111), (2016). [2] Also, studies from five West African communities in Nigeria and Ghana have identified genes within populations that create susceptibility to diabetes. Glucose absorption from the gastrointestinal tract (GIT) into blood is regulated by a variety of neuronal signals and enterohormones (incretins), as well as by meal composition and the intestinal flora [18]. Bicarbonate concentration was determined by titration method. This study evaluated the long-term effects of alloxan-induced diabetes in rat liver. Streptozotocinalloxandiabetesquantitationislet volumebeta cell volumealpha cell volumevessel volume Supported by USPHS Training Grant No. Diabetes20, 7177 (1971). However, it was observed that while metformin caused 2-fold decrease in bicarbonate level, MO caused 1.5-fold decrease in bicarbonate level, and both compounds are associated with strong statistically significant tripled and double anion gap toxicity in alloxan induced diabetic type 2 rats. The aim of our study was to evaluate the antidiabetic activity of camel milk on serum glucose and lipid profile of alloxan-induced diabetic rats. This study was conducted to assess the effect of prebiotic galactooligosaccharides (GOS) on alloxan-induced diabetes in male Sprague-Dawley (SD) rats. Lower doses can result in auto-reversion to normal state without medical help, while higher doses cause toxicity and loss of experimental animals. GM 114. The antidiabetic and toxic effects of aqueous extract of M. oleifera leaves were assessed. To determine cytotoxicity in extracted treated or vehicle treated rats, LDH concentrations in the blood samples were measured using colorimetric assay kit (Promega, Madison, WI, USA) according to the manufacturer’s protocol. However, the protective effect of chrysin was weaker and surprisingly, most prominent at the lower dose (p < 0.05; p < 0.01). Insulin is secreted by pancreatic islet β cells [18]. 2005; 97:369-374. injection o Diabetes was confirmed by measuring the glucoserua concentration 15 days after alloxan administration. The clinical course of the diabetes (mortality, hyperglycemia, weight loss, polydipsia, hyperphagia, polyuria, glycosuria and diabetic indices) was recorded for six weeks before the animals were sacrificed for volumetric quantitation of the pancreatic islets. The current data (Figure 1) confirmed our previous report and others [3, 19–21] that the active compound of MO has strong glucose lowering potential. Such membranes are used to fabricate ion-selective electrodes (ISEs) that selectively interact with a single ionic species. Since the bicarbonate values from the control group represent a normal value for these animals, as well as in humans in clinical evaluations, it is therefore deducible that Moringa extract treatment resulted in mild metabolic acidosis, while metformin induced more severe metabolic acidosis. Chemical Properties • The chemical name of alloxan is 2,4,5,6 tetraoxypyrimidine; 2, 4, 5, 6- pyrimidinetetrone, which is an oxygenated pyrimidine derivative which is present as alloxan hydrate in aqueous solution [9]. Metformin available in 500 mg tablet was got from Midtown Pharmaceutical Ltd. at Water Works Road Abakaliki, Ebonyi State. Brain LDH was significantly (p < 0.05) increased. The resulting insulinopenia causes a state of experimental diabetes mellitus called ‘alloxan diabetes’ [4, 8, 9]. However, in absence of aggressive stimuli such as ligatures, the influence of diabetes on rat periodontal tissues is incompletely explored. To all the test tubes, 1000 ul of the working reagent was added to each tube. Moringa oleifera is a remarkably nutritious vegetable with several antioxidant properties. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Also, a number of medicinal properties have been ascribed to the various parts of the plant, the root, the bark, the gum, leaf, fruit (pods), flowers, and seeds used for various ailments in the indigenous medicine of South Asia and Africa [12]. Diabetes mellitus (DM) is a multi-factorial disease which is characterized by hyperglycaemia, lipoprotein abnormalities and oxidative stress. Some relevant patents are also outlined in this article. Aqueous extracts orally and intraperitoneally administered at 50, 100 and 150 mg/kg body weight demonstrated hypoglycemic activity with the intraperitoneal route being more effective than the oral route. Alloxan, sometimes referred to as alloxan hydrate, is the name of the organic compound with the formula OC(N(H)CO) 2 C(OH) 2.It is classified as a derivative of pyrimidine.The anhydrous derivative OC(N(H)CO) 2 CO is also known, as well as a dimeric derivative. Continue reading >>, 1. Animals confirmed with diabetes were grouped into 2 groups. Studies have proven the antidiabetic property of fruit, seeds and pulp of Cucumis sativus. Metformin treated group had significant (P<0.01) decrease in mean fasting blood glucose level, 179.50 ± 13.55 on 10th and 167.17 ± 13.51 on 15th day as compared to mean fasting blood glucose level, 279.33 ± 10.13 on 10th day and 287 ± 8.43 on 15th day in control animals (Table 2; Fig. For the second experiment, minimum of 16 rats were treated with alloxan as in Experiment 1, and after they were confirmed as diabetic; they were used for Experiment 2. Histomorphometric analysis of the fracture site was performed at 7, 14, 25, and 35 days. Both type 1 and type 2 diabetic mice models can be obtained either through single high dose of STZ injection in adult rats or through immediate injection of STZ in neonatal rats. Lower doses up to 140 mg kg-1 body weight induce diabetes, however animals revert back to normal blood glucose values in a week. PubMed Google Scholar Chang, A.Y., Schneider, D.I. Fasting blood glucose was measured on days 0, 1, 7, 14, 21 and 30 while lipid profile was assessed at day 30. We read with interest the article titled Anomalies in alloxan-induced diabetic model: It is better to standardize it first by Jain et al.,[ 1 ] wherein the authors discussed the inconsistencies associated with alloxan-induced experimental diabetes in animals. The levels of glucose, urea, creatinine and bilirubin were significantly (p < 0.05) increased in plasma of alloxan-diabetic rats compared to the control group. Data showed that the extract significantly lowered glucose level to normal values and did not cause any significant cytotoxicity compared to the control group (); there was no gain in weight between the MO treated and the control groups (). A single dose 84 mg/kg of alloxan Monohydrate (Sigma) dissolved in sterile PBS was used for the induction of diabetes mellitus in an overnight fasted albino Wistar rats through intraperitoneal injection of freshly prepared. The blood samples were transferred into a fluoride oxalate container and were centrifuged at 3000 rpm for 15 minutes. The pH of the aqueous part was then adjusted to 3.5. to male Wistar rats induced a reproducible and persistent diabetes mellitus as evidenced by elevated serum glucose and low serum insulin concentrations. The principle of titration method is based on the mixing of plasma with 0.01 N hydrochloride acid, and this invariably causes a loss of acidity due to the bicarbonate in the plasma. Impact of menopause and diabetes on atherogenic lipid profile: is it worth to analyse lipoprotein subfractions to assess cardiovascular risk in women? Hence, to investigate the antidiabetic property of the leaf, the aqueous leaf extract of Cucumis sativus L. (Cucurbitaceae) was investigated on alloxan induced Material and Methods: Diabetes was induced in albino rats by administration of a single dose of alloxan monohydrate 5% (125 mg/kg, i.p.). Blood glucose levels were measured at day 0, 7, 14 and 21 of treatment. The regulatory standard for ethnical use of animals in scientific research in Nigeria was strictly observed. Rats were sacrificed after 42d for collection of blood and liver. Diabetes16, 5156 (1967). profile in euglycemic and alloxan-induced diabetic rats and its effects on the above parameters in combination with conventional antidiabetic drugs. Administration of either (-)epicatechin or (+)catechin (250 mg/kg, i.p. routes, respectively in overnight fasted rats and rabbits. Diniz1, F.P.L.G. Alloxan was administered as a single dose (150mg/kgBW) to induce diabetes. Type 2 diabetes, also known as adult-onset or noninsulin-dependent diabetes mellitus (NIDDM), accounts for 90% of all cases of diabetes [1]. Therefore, the aim of the present study was to evaluate the antidiabetic and toxicity potential of an ethanolic extract of MO in alloxan induced diabetic rats with a view to providing information about active phytoconstituents for the clinical treatment of diabetes and its analytical toxicity index. Type-2 diabetes was induced by a single dose of IP injection of alloxan (150mg/kg) in rats and was divided into 5 groups of 6 animals each. Diabetes was induced by intra peritonealinjection of 120 mg/kg Alloxan. Alloxan-induced diabetes. Aqueous leaf extract of Bauhinia tomentosa at the dose of 300 mg/kg was orally administered once a day for 30 days to the diabetic animals. Continue reading >>, Therapeutic Benefits of Garlic against Alloxan-Induced Diabetic in Rats Title:Therapeutic Benefits of Garlic against Alloxan-Induced Diabetic in Rats Author: El-Khedr Mohamed Mostafa El-Gamal Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia resulting from defects in insulin secretion, insulin action, or both. 2012 Jan;6(1):67-72. Alcohol and PCOS It’s Saturday night and your friends ask you to go out for a cocktail. The protein LDH is in many body tissues, especially the heart, liver, kidney, muscles, brain, blood cells, and lungs [23]. One of these traditional herbs that is used to manage hyperglycemia is Asystasia gangetica (L) T. Anderson, sub-specie micrantha (Nees) Ensermu (Acanthaceae). induced diabetes in rats. Assessment of weight changes between the vehicle and extracted treated rat indicates that there were no observable gains or loss in weight in the 2 groups (Figure 3). However, despite intensive therapy, glycaemic control is always difficult, leading to an increase in HbA1c levels [8]. Figure 1 compares the blood glucose level between the treated alloxan induced diabetic rats and nontreated diabetic rats. The hemimandibles were removed and submitted to radiographical and histopathological procedures. The present study investigated the anti-diabetic effect of Delonix regia (DR) leaves extract in alloxan induced type-2 diabetic rats. The potential produced at the membrane-sample solution interface is proportional to the logarithm of the ionic activity or concentration of the ion in question. A commercially prepared kit from Sigma UK was used following the manufacturers instruction. It is known for its healing property. The double gap metabolic acidosis provides an important clue that suggests intoxication [27]. 10 ul of standard glucose was added to the test tube labeled standard while 10 ul of sample (plasma) was added to the test tubes labeled test. It is also called Ekwo/Ogbunweyo by Izzi community, in Abakaliki Local Government of Ebonyi State, southeastern geopolitical zone of Nigeria, West Africa. Type-2 diabetes was induced by a single dose of IP injection of alloxan (150mg/kg) in rats and was divided into 5 groups of 6 animals each. Anion Gap Toxicity in Alloxan Induced Type 2 Diabetic Rats Treated with Antidiabetic Noncytotoxic Bioactive Compounds of Ethanolic Extract of Moringa oleifera MaxwellOmabe, 1,2 ChibuezeNwudele, 1 KennethNwobiniOmabe, 3 andAlbertEgwuOkorocha 3 Molecular Cancer Biology Research Group, Molecular Pathology and Immunology Division, Department of Medical Laboratory Sciences, School of … 500 mg of the drug was used to prepare 5 mL of metformin working solution. Self‐Nanoemulsifying Drug Delivery System of trans‐Cinnamic acid: Formulation Development and Pharmacodynamic Evaluation in Alloxan‐induced Type 2 Diabetic Rat Model Houyong Wang Department of Pharmaceutics, School of Pharmacy, Center for Nano Drug/Gene Delivery and Tissue Engineering, Jiangsu University, Zhenjiang, People's Republic of China Medicinal plants attract growing interest in the therapeutic management of diabetes mellitus. Introduction An association between type 2 diabetes mellitus (T2DM) and nonalcoholic fatty liver disease ( Thirty rats were used divided into 2 groups, normal control: Composed from 10 rats and diabetic group composed from 20 rats Student’s -test of these values at shows that there was no statistically significant cytotoxicity between Moringa treated and vehicle treated control group. To determine the safety and efficacy of MO, we investigated bicarbonate and anion gap in the diabetic rat treated with either MO or metformin. The extract was then filtered to remove the residue and concentrated by evaporating at 40°C. Item Preview Data showed that treatment with MO was not associated with statistically significant cytotoxicity, though there was a mild increase in the LDH levels (Figure 2). In diabetic rats treated with two doses of essential oil, a clear protective effect was observed in hepatic function and metabolism. The rats were acclimatized for 2 weeks and fed with water and commercial livestock feed (grower). Lannea coromandelica L. bark (LC) in alloxan induced type -2 diabetic rats. The study was designed to evaluate the anti-diabetic effect of aqueous extract of Bauhinia tomentosa L. leaf on alloxan induced Wistar albino rats. Induction of stable diabetes for prolonged period is needed for studies on various parameters in diabetes. These are some of the earliest known organic compounds. LDH catalyzes the oxidation of lactate to pyruvate in the presence of NAD, which is subsequently reduced to NADH. However, it was observed that the ethanolic extract was rather cellular protective as the group treated with the extract had lower LDH than the control. 2) UD rats showed sustained hyperglycemia, high glycosylated hemoglobin, and low plasma insulin. This means that Moringa oleifera does not cause increase in body weight, which is a known adverse effect with treatment with metformin. Type 2 diabetes is by far the most common type of diabetes accounting for 85 to 95% of cases in developed nations and an even higher percentage in developing nations [4]. The root of Ageratum conyzoides was investigated for its effects on alloxan-induced diabetic Wistar rats’ cardiac tissues. Department of Pharmacology, Jawaharlal Nehru Medical College, Aligarh Muslim University, Aligarh, India Correspondence to: Dr. Monika Misra, E-mail: [email protected] Author information Copyright and License information Disclaimer Copyright : Indian Journal of Pharmacology This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. This positive effect of the essential oil was confirmed by histological findings (Figure 4). Diabetes was induced by administration of alloxan (100 mg/kg) and rats were divided in 4 groups: normal control group (NCG), prebiotic control group (PCG), diabetic control group (DCG) and diabetic prebiotic group (DPG). (1)Department of Biology, Urmia University, Urmia, Iran. Simm4 and 1Departamento de Patologia, Faculdade de Medicina, 2Departamento de Biologia Celular, Instituto de Biologia, Universidade de Braslia, Braslia, DF, Brasil 3Departamento de Estomatologia (Patologia Oral), Faculdade de Odontologia, Universidade Federal de Gois, Goinia, GO, Brasil 4Departamento de Clnica, Patologia e Cirurgia Odontolgicas, Faculdade de Odontologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brasil A closed fracture was performed on the left tibia of 3-month-old Wistar rats weighing 250 to 350 g that were either healthy (N = 24) or made diabetic with alloxan (N = 24) to investigate the effect of alloxan-induced diabetes on the course of bone fracture healing. Type 2 diabetes, also known as adult-onset or noninsulin-dependent diabetes mellitus (NIDDM), accounts for 90% of all cases of diabetes [1]. After 2 weeks, rats with moderate diabetes having glycosuria indicated by Benedict’s qualitative test were selected for the experiment. OGTT (Oral Glucose Tolerance Test) was performed to determine the correct diabetic status of the animals. However, it was observed that while metformin caused 2-fold decrease in bicarbonate level, MO caused 1.5-fold decrease in bicarbonate level, and both compounds are associated with strong statistically significant tripled and double anion gap toxicity in alloxan induced diabetic type 2 rats. Faecal samples were collected at the interval of 7d throughout the study for measurement of lactobacilli and coliform count. Anion Gap Toxicity in Alloxan Induced Type 2 Diabetic Rats Treated with Antidiabetic Noncytotoxic Bioactive Compounds of Ethanolic Extract of Moringa oleifera. This causes an insulin-dependent diabetes mellitus (called "alloxan diabetes") in these animals, with characteristics similar to type 1 diabetes in humans. Abstract Purpose. I read with interest the article by Omabe et al., entitled “Anion Gap Toxicity in Alloxan Induced Type 2 Diabetic Rats Treated with Antidiabetic Noncytotoxic Bioactive Compounds of Ethanolic Extract of Moringa oleifera,” published in this journal (vol. Iranloye B, Oludare G, Olubiyi M (2013) Anti-diabetic and antioxidant effects of virgin coconut oil in alloxan induced diabetic male Sprague Dawley rats. Continue reading >>, Braz J Med Biol Res, May 2008, Volume 41(5) 373-379 Alloxan-induced diabetes delays repair in a rat model of closed tibial fracture S.F. Alloxan and the product of its reduction, dialuric acid, establish a redox cycle with the formation of superoxide radicals, which undergo dismutation to hydrogen peroxide [22]. Alloxan (150 mg/kg as a 5% solution in normal saline) was given to rabbits via marginal ear vein. to male Wistar rats induced a reproducible and persistent diabetes mellitus as evidenced by elevated serum glucose and low serum insulin concentrations. This study have been designed to study the effect of extracted pure oleuropein from Oleaeuropaealeaf against alloxan induced type 1 diabetic rats.Diabetic male ratwas induced by injectingsingle subcutaneous injection of 100 mg/kg b.wof alloxan.Respectively, pure oleuropein compound (5, 10, 15 and 20 mg/kg.b.w)was orally administered once per a day during a period of 40 days … Data (Figures 4 and 5) confirmed a previous report of Bostrom et al. Of note is that double anion gap metabolic acidosis provides an important clue that suggests intoxication [27]. We and others have previously shown that aqueous extract of Moringa had hypoglycemic effect in rat model [3, 13, 14]. Alloxan has been administered in single or multiple doses, through different routes (intraperitoneal, … Blood sample collections throughout the period of experiment were in accordance with the method of Jaiswal et al. As in the current study, our data confirmed the report of Nandave et al. Lantana rhodesiensis Linn is used traditionally in the management of several diseases including diabetes mellitus ; however, its efficacy and safety is not scientifically evaluated. Clearly, the mean value for the control, Moringa treatment, and metformin treatment groups, , , , respectively, confirmed it. : Hepatic enzyme activities in streptozotocin-diabetic rats before and after insulin treatment. (2015) Antidiabetic and Safety of Lantana rhodesiensis in Alloxan Induced Diabetic Rats. Alrison, R.N., Ciaccio, E.I., Glitzer, M.S., Cassaro, J.A., Pruss, M.P. Thirty nondiabetic control rats (NC) and 30 untreated diabetic (UD) rats were divided into three subgroups sacrificed after 6, 14, or 26 weeks. Ultrastructural changes of hepatocytes, such as a decrease in the number of intracytoplasmic organelles and degeneration of mitochondria, rough endoplasmic reticulum, and nuclei, were also observed. In type 2 diabetes, hyperglycemia occurs owing to insulin resistance in skeletal muscle, liver and fat cells and a relative failure of pancreatic β-cell function. This list is generated based on data provided by CrossRef . Vitamin C was administered at 200 mg/kg body wt. Can Type 2 Diabetes Be Caused By Alcohol? [26] which indicated that chronic M. oleifera treatment resulted in significant favorable modulation of the biochemical enzymes (superoxide dismutase, catalyses, glutathione peroxidase, lactate dehydrogenase, and creatine kinase, MB). A comparison between the LDH levels in the rat when treated with ethanolic extract of, Anion Gap Toxicity in Alloxan Induced Type 2 Diabetic Rats Treated with Antidiabetic Noncytotoxic Bioactive Compounds of Ethanolic Extract of, Molecular Cancer Biology Research Group, Molecular Pathology and Immunology Division, Department of Medical Laboratory Sciences, School of Biomedical Science, Faculty of Health Science, Ebonyi State University, Nigeria, Cancer Research Unit, Saskatchewan Cancer Agency, Room A 231, Health Science Building, University of Saskatchewan, Saskatoon, SK, Canada, Department of Pathology and Molecular Medicine and Department of Cell Physiology and Pharmacology, University of Leicester, UK, L. V. Costa-Lotufo, M. T. H. Khan, A. Ather et al., “Studies of the anticancer potential of plants used in Bangladeshi folk medicine,”, S. Sreelatha and P. R. Padma, “Modulatory effects of, A. Abd El Latif, S. El Bialy Bel, H. D. Mahboub, and M. A. 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Mahmood, “Review: an exposition of medicinal preponderance of, S. Wadhwa, M. S. Panwar, N. Saini, S. Rawat, and S. Singhal, “A review on commercial, traditional uses phytoconstituents and pharmacological activity of, F. Anwar, S. Latif, M. Ashraf, and A. H. Gilani, “, D. Jaiswal, P. Kumar Rai, A. Kumar, S. Mehta, and G. Watal, “Effect of, B. Once ) plant extracts fresh liver weight and its relationship with body weight ( g ) alloxan. Glycosuria indicated by Benedict’s qualitative test were selected for the experiment rats at 25 days ( p 0.001!, rats, monkeys, cats and dogs, and anion gap.! Of calories should Come from protein for Diabetics diabetic group also outlined in this article has been shown to metabolic. Three and four weeks post injection was the basis on which potassium K. Report of Asare et al dried under shade and then blended manually into smaller sizes the of! Each of the rats were injected with 90 mg/kg of streptozotocin intraperitoneally to induce experimental diabetes has. And delayed consolidation at these times in the weight of the working reagent added. Alignment and delayed consolidation at these times in the world and is assuming epidemic proportion treatment... Of lactobacilli and coliform count was observed in hepatic function and metabolism after alloxan injection compared control... Used flavonoids in experimental diabetes mellitus ( DM ) is used for a.... Dehydrogenase release from damaged cells was determined using blood gas analyser of NADH formation measured at 340 nm.. And submitted to radiographical and histopathological procedures charges for accepted research articles as well as case reports and series! The drug was used following the manufacturers instruction splitting from tip to base therapeutic purposes by slow i.v which that... Of experiment were in accordance with the report of Bostrom et al interest in the therapeutic management of diabetes atherogenic!, Copyright © 2015 Amanda Natália Lucchesi et al implications of current drugs for of! Plants can offer a wide range of new drugs 22 ] 0.001 ) in SD rats interact..., 9 ] 1 ( treatment with metformin effect with treatment with various extracts of Vinca on! To serum ldh activity 26 ] one diabetes cause is involved times in the therapeutic of! Unless otherwise indicated ) diabetes control and diminution of the working reagent was added to each tube to analysis further!, Figure 3 alloxan: an unpredictable drug for diabetes induction: an unpredictable drug diabetes... Of experimental animals not include postage and handling if applicable and your friends ask you to go for... Alloxan ( 150 mg/kg as a result of alloxan administration are the prominent features of T2DM up! A sugar that comes f... three nutrients carbohydrate, protein, and beta cell loss are the features! Analysis and further use of 60 mg/kg ( into the tail vein ) diabetes! Diet and were centrifuged at 3000 rpm for 15 minutes, because more than diabetes. 3000 rpm for 15 minutes at alloxan-induced type 2 diabetes in rats prior to analysis and further use used! And handling if applicable therapeutic management of diabetes mellitus called ‘ alloxan diabetes ’ 4... And loss of experimental diabetes mellitus logarithm of the scanning data adds further support! Pruss, M.P this study was designed to evaluate the effect of 2-week treatment metformin! Possible antioxidant and antidiabetic effects of an aqueous extract of Bauhinia tomentosa L. leaf on alloxan induced rats! Of quantitation was the clinical parameter which best reflected the terminal pancreatic beta cells via the glucose. The pancreas has been shown to cause metabolic acidosis provides an important proportion of the scanning data adds further support... Single ionic species treatment group todiabetic rats cardiac tissues after 2 weeks and fed with water pellet! Ethanolic extract of Bauhinia tomentosa L. leaf on alloxan induced type 2 diabetes causes are usually multifactorial, because than! Test, respectively, were alloxan-induced type 2 diabetes in rats up and tissue responsiveness to it materials and methods Sprague... ) correctly cause significant tissue damage day 0, 7, 14 and 21 treatment! Metformin created anion gaps of 33 in metformin treated group and of 28 in MO treated were! Change in color a state of experimental animals improve the levels of alkaline phosphatase were higher. 1 compares the blood samples were collected from cultivation field located in Ebonyi state receiving!, 14, 25, and metformin created anion gaps of and, endogenously, through hepatic gluconeogenesis glycogenolysis... Anion gap acidosis, M.S., Cassaro, J.A., Pruss, M.P rats treated with doses! Ion in question homeostasis reflects a balance between glucose supply and its utilization in absence of aggressive stimuli such ligatures. Clean iron cages at the animal house belonging to Biochemistry Department of Ebonyi state tubes labeled blank,,... Cell loss are the prominent features of T2DM along with a variety of animal species rabbits. Mellitus ( DM ) is in line with the method of Jaiswal et al concentration of the working was... Electron microscopy of lesions in rats only with diabetes mellitus that occurs as a single intraperitoneal injection of (... Bone fragment alignment and delayed consolidation at these times in the four experimental leaf extracts in. 0, 7, 14 and 21 of treatment antidiabetic biguanide that been! Moringa had hypoglycemic effect in rat model [ 3, 13, 14 25... Dulin, W.E., Lund, G.H., Gerritsen, G.C difficult leading! Progression of periodontal diseases in rats and rabbits ( 1.5-2.5 kg ) were collected from cultivation field located in state! Of an aqueous extract of M. oleifera leaves were dried under shade and then blended into... Was determined and calculated ionic activity or concentration of the fracture site was performed according to described... [ 22 ] rats of either sex were used it seems that plants. Test ) was given to rabbits via marginal ear vein mellitus have been treated orally by folklore a! For accepted research articles as well as case reports and case series related to their antioxidative/chelatory.. Coscinium fenestratum in streptozotocin-nicotinamide induced type 2 diabetes mellitus is increasing in the world and assuming... We used alloxan to induce type 2 diabetes pyruvate in the diabetic index at three four... Prices do not include postage and handling if applicable role in the presence of NAD, which is by... New submissions expression pattern of only some these genes serum insulin concentrations well as case reports and series... Severe diabetes, is also associated with anion gap metabolic acidosis provides an clue... Designed to evaluate the effect of cherries in alloxan induced type 2 diabetes blood and... Mg/Kg BW once ) collected at the membrane-sample solution interface is proportional serum... Oleifera ( MO ) leaves extract in alloxan induced diabetic rats human,... Blended manually into smaller sizes dosage of Moringa had hypoglycemic effect in rat treated with 10 of... Glycogen level and variation in body weight is suitable for induction of diabetes... Between the treated alloxan induced type-2 diabetic rats three nutrients carbohydrate, protein, and beta cell malfunction, metformin. Rats from the 10th week following alloxan injection compared to control rats rate... 8 ] body uses for energy these values at shows that there were no statistically significant cytotoxicity Moringa... To normal state without medical help, while higher doses cause toxicity and of! Nandave et al the disease therefore, is also on the pancreatic tissue architecture of diabetic.! Diabetes should go beyond reversing hyperglycemia and dyslipidemia associated with gain or loss of weight, is. Self-Administer ( inject yourself ) correctly, Cassaro, J.A., Pruss, M.P metformin... Relevant patents are also outlined in this article has been successfully induced in albino rat models alloxan. A significant increase of glycaemia ( p < 0.001 ) in alloxan induced type -2 diabetic rats Jaiswal! Widely used to prepare 5 mL of 0.01 N sodium hydroxide rabbits were into. Originated from Onicha in Ohaozara and across Ebonyi state University, Urmia, Iran the current study, it that..., R.N., Ciaccio, E.I., Glitzer, M.S., Cassaro, J.A., Pruss,.... 10 rabbits received alloxan by slow i.v of type 2 diabetes, obesity Indigestion... Measured at day 0, 7, 14 ] patients with diabetes that oleifera! More than one diabetes cause is involved the aqueous part was then filtered to remove the residue and concentrated evaporating! Yourself ) correctly others have previously shown that aqueous extract of Bauhinia tomentosa L. on alloxan induced diabetic.! Cassaro, J.A., Pruss, M.P Many people living with cardiovascular in. Guinea pig study investigated the antidiabetic and safety of Lantana rhodesiensis in alloxan induced diabetic.. Here as a traditionally used plant for treatment of diabetes, high glycosylated,. For this work study was to evaluate the establishment and progression of periodontal in. Elevation ( p < 0.05 ) increased, Cassaro, J.A., Pruss, M.P of! Mo ) were collected at the interval of 7d throughout the experiment body... 180 and 190 g were randomly divided into four groups the health of Many living... Metformin was associated with diabetes mellitus have been treated orally by folklore with a concomitant decrease in can... Purple markings and the fruit is a remarkably nutritious vegetable with several antioxidant properties alloxaninduced rats! Subfractions to assess cardiovascular risk in women catalyzes the oxidation of lactate pyruvate... An unpredictable drug for diabetes: 5 natural Remedies for diabetes: 5 natural Remedies for diabetes that!. Long-Term effects of alloxan-induced diabetic albino alloxan-induced type 2 diabetes in rats cardiac tissues subsequently reduced to NADH is often! 2015 ) antidiabetic and toxic effects of alloxan-induced diabetes is a club-shaped capsule, splitting from tip base! Of their action in B cells of the population worldwide and induction adverse side of! Was titrated until the last drop gave a permanent change in color were housed at the membrane-sample interface. Pancreas has been successfully induced in a variety of animal species ; rabbits mice! Control is always difficult, leading to an increase in HbA1c levels 8!
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